goat anti gfap (Novus Biologicals)
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Goat Anti Gfap, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/goat+anti+gfap/GFAP+Antibody/pm40270006-71-60-64
Average 93 stars, based on 38 article reviews
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Immunostaining:Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood-brain barrier dysfunction after subarachnoid hemorrhage in mice. Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood–brain barrier dysfunction after subarachnoid hemorrhage in mice Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), Blocking Assay:Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood-brain barrier dysfunction after subarachnoid hemorrhage in mice. Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood–brain barrier dysfunction after subarachnoid hemorrhage in mice Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), Staining:Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood-brain barrier dysfunction after subarachnoid hemorrhage in mice. Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood–brain barrier dysfunction after subarachnoid hemorrhage in mice Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), Incubation:Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood-brain barrier dysfunction after subarachnoid hemorrhage in mice. Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood–brain barrier dysfunction after subarachnoid hemorrhage in mice Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), Immunofluorescence:Article Title: Organotypic culture of human brain explants as a preclinical model for AI-driven antiviral studies. Article Snippet: .. The following antibodies were used for immunofluorescence in this study: rabbit polyclonal anti-MAP2 (1/100, GeneTex, #GTX133109), rat anti-CTIP2 (1/100, BioLegend, clone [25B6]), Article Title: Organotypic culture of human brain explants as a preclinical model for AI-driven antiviral studies Article Snippet: .. The following antibodies were used for immunofluorescence in this study: rabbit polyclonal anti-MAP2 (1/100, GeneTex, #GTX133109), rat anti-CTIP2 (1/100, BioLegend, clone [25B6]), other:Article Title: Deficiency of astrocyte CysLT 1 R ameliorates depression-like behaviors in mice by modulating glutamate synaptic transmission. Article Snippet: The following antibodies were used: mouse anti-GLT-1 (SC-365634) from Santa Cruz Biotechnology (Beverly, MA, USA); rabbit anti-PSD-95 (2507S), rabbit anti-SYN (5461S), rabbit anti-GluN2A (4205S), rabbit anti-GluN2B (14544S), rabbit anti-GluA1 (13185S), rabbit anti-GluA2 (5306S), rabbit anti-NF-κB p65 (8242S) from Cell Signaling Technology (Boston, MA, USA); rabbit anti-β-actin (AP0060) and rabbit antiLamin B1 (BS3547P) from Biowold Technology (Beijing, China); rabbit anti-CysLT1R (LS-C801414) from LifeSpan Bioscience (Seattle, WA, USA); chicken anti-NeuN (ABN91) from Sigma-Aldrich (St. Louis, Missouri, USA); |
