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goat anti gfap  (Novus Biologicals)


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    Structured Review

    Novus Biologicals goat anti gfap
    Goat Anti Gfap, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+anti+gfap/GFAP+Antibody/pm40270006-71-60-64
    Average 93 stars, based on 38 article reviews
    goat anti gfap - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Immunostaining:

    Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood-brain barrier dysfunction after subarachnoid hemorrhage in mice.
    Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), goat anti-GFAP (1:400, NB100-53809, Novus Biologicals, Shanghai, China), rabbit anti-CXCL12 (1:200, YT4225, Immunoway), rabbit anti-CD8 (1:100, 0108-7, HUABIO, Hangzhou, China), rabbit anti-CD4 (1:200, YT0762, Immunoway), mouse anti-CXCR4 (1:400, sc-54534, Santa Cruz Biotechnology, Shanghai, China), and rabbit anti-ZO. ..

    Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood–brain barrier dysfunction after subarachnoid hemorrhage in mice
    Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), goat anti-GFAP (1:400, NB100-53809, Novus Biologicals, Shanghai, China), rabbit anti-CXCL12 (1:200, YT4225, Immunoway), rabbit anti-CD8 (1:100, 0108-7, HUABIO, Hangzhou, China), rabbit anti-CD4 (1:200, YT0762, Immunoway), mouse anti-CXCR4 (1:400, sc-54534, Santa Cruz Biotechnology, Shanghai, China), and rabbit anti-ZO. ..

    Blocking Assay:

    Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood-brain barrier dysfunction after subarachnoid hemorrhage in mice.
    Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), goat anti-GFAP (1:400, NB100-53809, Novus Biologicals, Shanghai, China), rabbit anti-CXCL12 (1:200, YT4225, Immunoway), rabbit anti-CD8 (1:100, 0108-7, HUABIO, Hangzhou, China), rabbit anti-CD4 (1:200, YT0762, Immunoway), mouse anti-CXCR4 (1:400, sc-54534, Santa Cruz Biotechnology, Shanghai, China), and rabbit anti-ZO. ..

    Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood–brain barrier dysfunction after subarachnoid hemorrhage in mice
    Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), goat anti-GFAP (1:400, NB100-53809, Novus Biologicals, Shanghai, China), rabbit anti-CXCL12 (1:200, YT4225, Immunoway), rabbit anti-CD8 (1:100, 0108-7, HUABIO, Hangzhou, China), rabbit anti-CD4 (1:200, YT0762, Immunoway), mouse anti-CXCR4 (1:400, sc-54534, Santa Cruz Biotechnology, Shanghai, China), and rabbit anti-ZO. ..

    Staining:

    Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood-brain barrier dysfunction after subarachnoid hemorrhage in mice.
    Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), goat anti-GFAP (1:400, NB100-53809, Novus Biologicals, Shanghai, China), rabbit anti-CXCL12 (1:200, YT4225, Immunoway), rabbit anti-CD8 (1:100, 0108-7, HUABIO, Hangzhou, China), rabbit anti-CD4 (1:200, YT0762, Immunoway), mouse anti-CXCR4 (1:400, sc-54534, Santa Cruz Biotechnology, Shanghai, China), and rabbit anti-ZO. ..

    Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood–brain barrier dysfunction after subarachnoid hemorrhage in mice
    Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), goat anti-GFAP (1:400, NB100-53809, Novus Biologicals, Shanghai, China), rabbit anti-CXCL12 (1:200, YT4225, Immunoway), rabbit anti-CD8 (1:100, 0108-7, HUABIO, Hangzhou, China), rabbit anti-CD4 (1:200, YT0762, Immunoway), mouse anti-CXCR4 (1:400, sc-54534, Santa Cruz Biotechnology, Shanghai, China), and rabbit anti-ZO. ..

    Incubation:

    Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood-brain barrier dysfunction after subarachnoid hemorrhage in mice.
    Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), goat anti-GFAP (1:400, NB100-53809, Novus Biologicals, Shanghai, China), rabbit anti-CXCL12 (1:200, YT4225, Immunoway), rabbit anti-CD8 (1:100, 0108-7, HUABIO, Hangzhou, China), rabbit anti-CD4 (1:200, YT0762, Immunoway), mouse anti-CXCR4 (1:400, sc-54534, Santa Cruz Biotechnology, Shanghai, China), and rabbit anti-ZO. ..

    Article Title: Single-cell sequencing reveals intracranial microvasculature-derived CXCL12 promotes CD8 + T-cell infiltration and blood–brain barrier dysfunction after subarachnoid hemorrhage in mice
    Article Snippet: Dehydrated brain tissues were embedded and sectioned at a thickness of 30 μm via a cryostat (CM3050S-3–1-1, Leica, Shanghai, China). .. The sections were permeabilized in Enhanced Immunostaining Permeabilization Buffer (P0097, P400 Biotechnology, Shanghai, China) for 30 min, blocked with QuickBlockTM Blocking Buffer for Immunol Staining (P0260, Beyotime) for 30 min, and incubated overnight at 4 °C with the following primary antibodies: mouse anti-CD31 (1:200, YT0752, Immunoway, Suzhou, China), rat anti-CD13 (1:300, MA1-33449, Invitrogen), mouse anti-Iba-1 (1:500, GB12105, ServiceBio, Wuhan, China), goat anti-GFAP (1:400, NB100-53809, Novus Biologicals, Shanghai, China), rabbit anti-CXCL12 (1:200, YT4225, Immunoway), rabbit anti-CD8 (1:100, 0108-7, HUABIO, Hangzhou, China), rabbit anti-CD4 (1:200, YT0762, Immunoway), mouse anti-CXCR4 (1:400, sc-54534, Santa Cruz Biotechnology, Shanghai, China), and rabbit anti-ZO. ..

    Immunofluorescence:

    Article Title: Organotypic culture of human brain explants as a preclinical model for AI-driven antiviral studies.
    Article Snippet: .. The following antibodies were used for immunofluorescence in this study: rabbit polyclonal anti-MAP2 (1/100, GeneTex, #GTX133109), rat anti-CTIP2 (1/100, BioLegend, clone [25B6]), goat anti-GFAP (1/100, Novus Biologicals, #NB100-53809), mouse anti-Tuj1 (1/100, Genetex, #GTX631836), rabbit anti-Iba1 (1/100, Genetex, #GTX101495), mouse anti-CD31 (1/100, PECAM, Miltenyi Biotec, #130-108-038), mouse anti-Bassoon (1/50, Abcam, clone [SAP7F407]), Rabbit anti-Homer1 (1/50, Synaptic system, #160 003), Rabbit anti-LPHN3 (1/100, ThermoFisher, #PA533908), Goat anti-FLRT3 (1/50, R&D Systems, #AF2795), rabbit antiCleaved Caspase-3 (1/100, Cell Signaling Technology, clone [5A1E]), goat anti-SOX9 (1/50, R&D Systems, AF3075). .. The following antibodies were used for histology examination: Mouse anti-NeuN (1/400, clone A60, #MAB377, Millipore), rabbit antiOligo2 (1/100, clone EP112, #BSB2562, BioSB/diagomics), Mouse anti-GFAP (1/400, clone 6F2, #MO761, DAKO), Rabbit anti-CD3 (#05278422001, Roche), mouse anti-CD68 (clone KP1, #M0814, DAKO).

    Article Title: Organotypic culture of human brain explants as a preclinical model for AI-driven antiviral studies
    Article Snippet: .. The following antibodies were used for immunofluorescence in this study: rabbit polyclonal anti-MAP2 (1/100, GeneTex, #GTX133109), rat anti-CTIP2 (1/100, BioLegend, clone [25B6]), goat anti-GFAP (1/100, Novus Biologicals, #NB100-53809), mouse anti-Tuj1 (1/100, Genetex, #GTX631836), rabbit anti-Iba1 (1/100, Genetex, #GTX101495), mouse anti-CD31 (1/100, PECAM, Miltenyi Biotec, #130-108-038), mouse anti-Bassoon (1/50, Abcam, clone [SAP7F407]), Rabbit anti-Homer1 (1/50, Synaptic system, #160 003), Rabbit anti-LPHN3 (1/100, ThermoFisher, #PA533908), Goat anti-FLRT3 (1/50, R&D Systems, #AF2795), rabbit anti-Cleaved Caspase-3 (1/100, Cell Signaling Technology, clone [5A1E]), goat anti-SOX9 (1/50, R&D Systems, AF3075). .. The following antibodies were used for histology examination: Mouse anti-NeuN (1/400, clone A60, #MAB377, Millipore), rabbit anti-Oligo2 (1/100, clone EP112, #BSB2562, BioSB/diagomics), Mouse anti-GFAP (1/400, clone 6F2, #MO761, DAKO), Rabbit anti-CD3 (#05278422001, Roche), mouse anti-CD68 (clone KP1, #M0814, DAKO).

    other:

    Article Title: Deficiency of astrocyte CysLT 1 R ameliorates depression-like behaviors in mice by modulating glutamate synaptic transmission.
    Article Snippet: The following antibodies were used: mouse anti-GLT-1 (SC-365634) from Santa Cruz Biotechnology (Beverly, MA, USA); rabbit anti-PSD-95 (2507S), rabbit anti-SYN (5461S), rabbit anti-GluN2A (4205S), rabbit anti-GluN2B (14544S), rabbit anti-GluA1 (13185S), rabbit anti-GluA2 (5306S), rabbit anti-NF-κB p65 (8242S) from Cell Signaling Technology (Boston, MA, USA); rabbit anti-β-actin (AP0060) and rabbit antiLamin B1 (BS3547P) from Biowold Technology (Beijing, China); rabbit anti-CysLT1R (LS-C801414) from LifeSpan Bioscience (Seattle, WA, USA); chicken anti-NeuN (ABN91) from Sigma-Aldrich (St. Louis, Missouri, USA); goat anti-GFAP (NB100–53809) from Novus Biologicals (Littleton, Colorado, USA); goat anti-Iba-1 (011–27,991) from Wako Pure Chemical (Kyoto, Japan).



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    BPAF reduced the number of neurons and injured synaptic growth. ( A ) Representative immunofluorescence images of ReNcell CX cells stained with the newborn neuronal marker doublecortin (DCX, Green), while the nuclear stain was DAPI (Hoechst 33342, blue) (Scale bar, 200 μm). ( C ) Sholl analysis of the number of intersections of radial distance from the cell body (N = 30 cells). ( D ) Representative immunofluorescence images of ReNcell CX cells stained with the astrocytes marker <t>(GFAP),</t> and the nuclei were counterstained by DAPI (blue) (Scale bar, 200 μm). ( B , E ) The percentage of immunofluorescence-positive cells. One-way ANOVA was applied to test the difference between two or more groups; two-way ANOVA and Bonferroni post-test were applied for the results with Sholl analysis. The data presented here represent the mean ± SD of at least three separate experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 when compared to the corresponding control group.
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    Image Search Results


    BPAF reduced the number of neurons and injured synaptic growth. ( A ) Representative immunofluorescence images of ReNcell CX cells stained with the newborn neuronal marker doublecortin (DCX, Green), while the nuclear stain was DAPI (Hoechst 33342, blue) (Scale bar, 200 μm). ( C ) Sholl analysis of the number of intersections of radial distance from the cell body (N = 30 cells). ( D ) Representative immunofluorescence images of ReNcell CX cells stained with the astrocytes marker (GFAP), and the nuclei were counterstained by DAPI (blue) (Scale bar, 200 μm). ( B , E ) The percentage of immunofluorescence-positive cells. One-way ANOVA was applied to test the difference between two or more groups; two-way ANOVA and Bonferroni post-test were applied for the results with Sholl analysis. The data presented here represent the mean ± SD of at least three separate experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 when compared to the corresponding control group.

    Journal: International Journal of Molecular Sciences

    Article Title: Bisphenol AF Induced Neurodevelopmental Toxicity of Human Neural Progenitor Cells via Nrf2/HO-1 Pathway

    doi: 10.3390/ijms26125685

    Figure Lengend Snippet: BPAF reduced the number of neurons and injured synaptic growth. ( A ) Representative immunofluorescence images of ReNcell CX cells stained with the newborn neuronal marker doublecortin (DCX, Green), while the nuclear stain was DAPI (Hoechst 33342, blue) (Scale bar, 200 μm). ( C ) Sholl analysis of the number of intersections of radial distance from the cell body (N = 30 cells). ( D ) Representative immunofluorescence images of ReNcell CX cells stained with the astrocytes marker (GFAP), and the nuclei were counterstained by DAPI (blue) (Scale bar, 200 μm). ( B , E ) The percentage of immunofluorescence-positive cells. One-way ANOVA was applied to test the difference between two or more groups; two-way ANOVA and Bonferroni post-test were applied for the results with Sholl analysis. The data presented here represent the mean ± SD of at least three separate experiments. * p < 0.05, ** p < 0.01, and *** p < 0.001 when compared to the corresponding control group.

    Article Snippet: The primary and secondary antibodies are anti-rabbit DCX, anti-mouse GFAP (1:200, Cell Signaling Technology, Danvers, MA, USA), and Alexa Fluor GFAP-labeled goat anti-rabbit antibody (1:200, Beyotime, Haimen, China), respectively.

    Techniques: Immunofluorescence, Staining, Marker, Control